Statistical modelling/Sample size estimation

We can now insert different numbers in the formula to simulate different replications.

Thus, suppose we used only 10 fields.



Suppose only 5 fields.

Naturally the SED increases when reducing the number of fields, although the increase is not very great. By calculating the least significant difference between two means (LSD = 2 x SED at the 95% level) it is possible that fewer fields of view per slide could have been sampled to achieve the same experimental result.